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EXPERIMENTAL MODEL AND SUBJECT DETAILS

Journal: Neuron

Article Title: Somatosensory Cortex Plays an Essential Role in Forelimb Motor Adaptation in Mice

doi: 10.1016/j.neuron.2017.02.049

Figure Lengend Snippet: EXPERIMENTAL MODEL AND SUBJECT DETAILS

Article Snippet: A subset of mice (n = 2) in this study was first tested in a similar adaptation task (unpublished data) prior to participating in this study (but in no more than 8 sessions). table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Organisms/Strains Mouse: C57BL/6J The Jackson Laboratory Jax # 000664 Mouse: B6.Cg-Tg(Slc32a1COP4*H134R/EYFP)8Gfng/J The Jackson Laboratory Jax # 014548 Mouse: B6.SJL- Slc6a3 tm1.1(cre)Bkmn /J The Jackson Laboratory Jax # 006660 Mouse: B6.Cg-Tg(Thy1-YFP)HJrs/J The Jackson Laboratory Jax # 003782 Software and Algorithms MATLAB (versions used: 2012b 2014a) Mathworks http://www.mathworks.com Python Python https://www.python.org/ Prism 5 Graph Pad Software http://www.graphpad.com LabView 2013 National Instruments http://www.ni.com MClust software A. David Redish http://redishlab.neuroscience.umn.edu/MClust/MClust.html Other Isoflurane (for anesthesia) IsoSol Vedco N/A Ketoprofen (for analgesia) Patterson Veterinary Cat #07-803-7389 Buprenorphine Patterson Veterinary Cat #07-850-2280 Dexamethasone Patterson Veterinary Cat #07-808-8194 Joystick base Digi-Key Cat #679-2501-ND Solenoid magnet McMaster-Carr Cat #69905K25 FlexiForce System: ELF 2 System Tekscan Cat # {"type":"entrez-nucleotide","attrs":{"text":"A00675","term_id":"14589"}} A00675 Mouse platform grip: thin rubber McMaster-Carr Cat #8633K52 NI-DAQ card, PCI-e6251 National Instruments Cat # NI PCIe-6251 – 779512-01 Laser for optogenetics: Opto Engine 473 nm −100 mW Opto Engine Cat # MBL-III473/1~100 mW Fine tetrode wire (nichrome) Sandvik Kanthal Cat # PF000591 Digital Lynx 4SX Recording System Neuralynx N/A Open Ephys Acquisition Board and Intan headstage Open Ephys http://www.open-ephys.org/store/starter-kit NeuroNexus 8 shank probe (with tetrode configuration) NeuroNexus N/A Open in a separate window EXPERIMENTAL MODEL AND SUBJECT DETAILS

Techniques: Software, Optogenetics

EXPERIMENTAL MODEL AND SUBJECT DETAILS

Journal: Neuron

Article Title: Somatosensory Cortex Plays an Essential Role in Forelimb Motor Adaptation in Mice

doi: 10.1016/j.neuron.2017.02.049

Figure Lengend Snippet: EXPERIMENTAL MODEL AND SUBJECT DETAILS

Article Snippet: A subset of mice (n = 2) in this study was first tested in a similar adaptation task (unpublished data) prior to participating in this study (but in no more than 8 sessions). table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Organisms/Strains Mouse: C57BL/6J The Jackson Laboratory Jax # 000664 Mouse: B6.Cg-Tg(Slc32a1COP4*H134R/EYFP)8Gfng/J The Jackson Laboratory Jax # 014548 Mouse: B6.SJL- Slc6a3 tm1.1(cre)Bkmn /J The Jackson Laboratory Jax # 006660 Mouse: B6.Cg-Tg(Thy1-YFP)HJrs/J The Jackson Laboratory Jax # 003782 Software and Algorithms MATLAB (versions used: 2012b 2014a) Mathworks http://www.mathworks.com Python Python https://www.python.org/ Prism 5 Graph Pad Software http://www.graphpad.com LabView 2013 National Instruments http://www.ni.com MClust software A. David Redish http://redishlab.neuroscience.umn.edu/MClust/MClust.html Other Isoflurane (for anesthesia) IsoSol Vedco N/A Ketoprofen (for analgesia) Patterson Veterinary Cat #07-803-7389 Buprenorphine Patterson Veterinary Cat #07-850-2280 Dexamethasone Patterson Veterinary Cat #07-808-8194 Joystick base Digi-Key Cat #679-2501-ND Solenoid magnet McMaster-Carr Cat #69905K25 FlexiForce System: ELF 2 System Tekscan Cat # {"type":"entrez-nucleotide","attrs":{"text":"A00675","term_id":"14589"}} A00675 Mouse platform grip: thin rubber McMaster-Carr Cat #8633K52 NI-DAQ card, PCI-e6251 National Instruments Cat # NI PCIe-6251 – 779512-01 Laser for optogenetics: Opto Engine 473 nm −100 mW Opto Engine Cat # MBL-III473/1~100 mW Fine tetrode wire (nichrome) Sandvik Kanthal Cat # PF000591 Digital Lynx 4SX Recording System Neuralynx N/A Open Ephys Acquisition Board and Intan headstage Open Ephys http://www.open-ephys.org/store/starter-kit NeuroNexus 8 shank probe (with tetrode configuration) NeuroNexus N/A Open in a separate window EXPERIMENTAL MODEL AND SUBJECT DETAILS

Techniques: Software, Optogenetics

EXPERIMENTAL MODEL AND SUBJECT DETAILS

Journal: Neuron

Article Title: Somatosensory Cortex Plays an Essential Role in Forelimb Motor Adaptation in Mice

doi: 10.1016/j.neuron.2017.02.049

Figure Lengend Snippet: EXPERIMENTAL MODEL AND SUBJECT DETAILS

Article Snippet: A subset of mice (n = 2) in this study was first tested in a similar adaptation task (unpublished data) prior to participating in this study (but in no more than 8 sessions). table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Organisms/Strains Mouse: C57BL/6J The Jackson Laboratory Jax # 000664 Mouse: B6.Cg-Tg(Slc32a1COP4*H134R/EYFP)8Gfng/J The Jackson Laboratory Jax # 014548 Mouse: B6.SJL- Slc6a3 tm1.1(cre)Bkmn /J The Jackson Laboratory Jax # 006660 Mouse: B6.Cg-Tg(Thy1-YFP)HJrs/J The Jackson Laboratory Jax # 003782 Software and Algorithms MATLAB (versions used: 2012b 2014a) Mathworks http://www.mathworks.com Python Python https://www.python.org/ Prism 5 Graph Pad Software http://www.graphpad.com LabView 2013 National Instruments http://www.ni.com MClust software A. David Redish http://redishlab.neuroscience.umn.edu/MClust/MClust.html Other Isoflurane (for anesthesia) IsoSol Vedco N/A Ketoprofen (for analgesia) Patterson Veterinary Cat #07-803-7389 Buprenorphine Patterson Veterinary Cat #07-850-2280 Dexamethasone Patterson Veterinary Cat #07-808-8194 Joystick base Digi-Key Cat #679-2501-ND Solenoid magnet McMaster-Carr Cat #69905K25 FlexiForce System: ELF 2 System Tekscan Cat # {"type":"entrez-nucleotide","attrs":{"text":"A00675","term_id":"14589"}} A00675 Mouse platform grip: thin rubber McMaster-Carr Cat #8633K52 NI-DAQ card, PCI-e6251 National Instruments Cat # NI PCIe-6251 – 779512-01 Laser for optogenetics: Opto Engine 473 nm −100 mW Opto Engine Cat # MBL-III473/1~100 mW Fine tetrode wire (nichrome) Sandvik Kanthal Cat # PF000591 Digital Lynx 4SX Recording System Neuralynx N/A Open Ephys Acquisition Board and Intan headstage Open Ephys http://www.open-ephys.org/store/starter-kit NeuroNexus 8 shank probe (with tetrode configuration) NeuroNexus N/A Open in a separate window EXPERIMENTAL MODEL AND SUBJECT DETAILS

Techniques: Software, Optogenetics

EXPERIMENTAL MODEL AND SUBJECT DETAILS

Journal: Neuron

Article Title: Somatosensory Cortex Plays an Essential Role in Forelimb Motor Adaptation in Mice

doi: 10.1016/j.neuron.2017.02.049

Figure Lengend Snippet: EXPERIMENTAL MODEL AND SUBJECT DETAILS

Article Snippet: A subset of mice (n = 2) in this study was first tested in a similar adaptation task (unpublished data) prior to participating in this study (but in no more than 8 sessions). table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Organisms/Strains Mouse: C57BL/6J The Jackson Laboratory Jax # 000664 Mouse: B6.Cg-Tg(Slc32a1COP4*H134R/EYFP)8Gfng/J The Jackson Laboratory Jax # 014548 Mouse: B6.SJL- Slc6a3 tm1.1(cre)Bkmn /J The Jackson Laboratory Jax # 006660 Mouse: B6.Cg-Tg(Thy1-YFP)HJrs/J The Jackson Laboratory Jax # 003782 Software and Algorithms MATLAB (versions used: 2012b 2014a) Mathworks http://www.mathworks.com Python Python https://www.python.org/ Prism 5 Graph Pad Software http://www.graphpad.com LabView 2013 National Instruments http://www.ni.com MClust software A. David Redish http://redishlab.neuroscience.umn.edu/MClust/MClust.html Other Isoflurane (for anesthesia) IsoSol Vedco N/A Ketoprofen (for analgesia) Patterson Veterinary Cat #07-803-7389 Buprenorphine Patterson Veterinary Cat #07-850-2280 Dexamethasone Patterson Veterinary Cat #07-808-8194 Joystick base Digi-Key Cat #679-2501-ND Solenoid magnet McMaster-Carr Cat #69905K25 FlexiForce System: ELF 2 System Tekscan Cat # {"type":"entrez-nucleotide","attrs":{"text":"A00675","term_id":"14589"}} A00675 Mouse platform grip: thin rubber McMaster-Carr Cat #8633K52 NI-DAQ card, PCI-e6251 National Instruments Cat # NI PCIe-6251 – 779512-01 Laser for optogenetics: Opto Engine 473 nm −100 mW Opto Engine Cat # MBL-III473/1~100 mW Fine tetrode wire (nichrome) Sandvik Kanthal Cat # PF000591 Digital Lynx 4SX Recording System Neuralynx N/A Open Ephys Acquisition Board and Intan headstage Open Ephys http://www.open-ephys.org/store/starter-kit NeuroNexus 8 shank probe (with tetrode configuration) NeuroNexus N/A Open in a separate window EXPERIMENTAL MODEL AND SUBJECT DETAILS

Techniques: Software, Optogenetics

EXPERIMENTAL MODEL AND SUBJECT DETAILS

Journal: Neuron

Article Title: Somatosensory Cortex Plays an Essential Role in Forelimb Motor Adaptation in Mice

doi: 10.1016/j.neuron.2017.02.049

Figure Lengend Snippet: EXPERIMENTAL MODEL AND SUBJECT DETAILS

Article Snippet: A subset of mice (n = 2) in this study was first tested in a similar adaptation task (unpublished data) prior to participating in this study (but in no more than 8 sessions). table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Organisms/Strains Mouse: C57BL/6J The Jackson Laboratory Jax # 000664 Mouse: B6.Cg-Tg(Slc32a1COP4*H134R/EYFP)8Gfng/J The Jackson Laboratory Jax # 014548 Mouse: B6.SJL- Slc6a3 tm1.1(cre)Bkmn /J The Jackson Laboratory Jax # 006660 Mouse: B6.Cg-Tg(Thy1-YFP)HJrs/J The Jackson Laboratory Jax # 003782 Software and Algorithms MATLAB (versions used: 2012b 2014a) Mathworks http://www.mathworks.com Python Python https://www.python.org/ Prism 5 Graph Pad Software http://www.graphpad.com LabView 2013 National Instruments http://www.ni.com MClust software A. David Redish http://redishlab.neuroscience.umn.edu/MClust/MClust.html Other Isoflurane (for anesthesia) IsoSol Vedco N/A Ketoprofen (for analgesia) Patterson Veterinary Cat #07-803-7389 Buprenorphine Patterson Veterinary Cat #07-850-2280 Dexamethasone Patterson Veterinary Cat #07-808-8194 Joystick base Digi-Key Cat #679-2501-ND Solenoid magnet McMaster-Carr Cat #69905K25 FlexiForce System: ELF 2 System Tekscan Cat # {"type":"entrez-nucleotide","attrs":{"text":"A00675","term_id":"14589"}} A00675 Mouse platform grip: thin rubber McMaster-Carr Cat #8633K52 NI-DAQ card, PCI-e6251 National Instruments Cat # NI PCIe-6251 – 779512-01 Laser for optogenetics: Opto Engine 473 nm −100 mW Opto Engine Cat # MBL-III473/1~100 mW Fine tetrode wire (nichrome) Sandvik Kanthal Cat # PF000591 Digital Lynx 4SX Recording System Neuralynx N/A Open Ephys Acquisition Board and Intan headstage Open Ephys http://www.open-ephys.org/store/starter-kit NeuroNexus 8 shank probe (with tetrode configuration) NeuroNexus N/A Open in a separate window EXPERIMENTAL MODEL AND SUBJECT DETAILS

Techniques: Software, Optogenetics